Figure 4

L-selectin increases cardiac mesoangioblast migration and has synergistic effects with SDF-1 pretreatment. (a) Cardiac mesoangioblasts transfected with one or two vectors expressing different surface molecules and EGFP were plated on endothelium-coated filters and induced to migrate for 6 h in the presence of mature cardiomyocytes in the lower chamber. A representative out of five independent experiments run in duplicate is shown (left panel) (left; ^*α<0.01). A representative image of the transmigrated cardiac mesoangioblasts transfected with L-selectin is also shown (right). Bar, 30 μm. (b) Cardiac mesoangioblasts transfected with vectors expressing L-selectin and/or β2-integrin and GFP were injected into the LV chamber of CAL mice, and after 6 h, the heart and filter organs were collected. Percentage of migrated cells was calculated after performing real-time PCR for GFP. A mean of three independent experiments run in triplicate is shown (^*α<0.02). (c) SDF-1-pretreated cardiac mesoangioblasts, transfected or not with vectors expressing L-selectin and GFP, were induced to migrate for 6 h through endothelium-coated filters and in the presence of mature cardiomyocytes in the lower chamber. A mean of three independent experiments run in duplicate is shown (left; ^*α<0.01). A representative image of the transmigrated SDF-1-pretreated cardiac mesoangioblasts transfected with L-selectin is also shown (right). Bar, 30 μm. (d) SDF-1-pretreated cardiac mesoangioblasts, transfected or not with vectors expressing L-selectin-EGFP, as well as control cardiac mesoangioblasts were injected into the LV chamber of CAL mice, and after 6 h, the heart and filter organs were collected and analyzed by real-time PCR for the presence of GFP. A mean of six independent experiments run in triplicate is shown (^*α<0.01)