Figure 6

Pretreatment of human cardiac mesoangioblasts also improves homing to the heart. (a) Human cardiac mesoangioblasts pretreated with SDF-1 or transfected with L-selectin construction were plated on endothelium-coated filters and induced to migrate for 6 h in the presence of cardiomyocytes. One representative out of three independent experiments run in duplicate is shown. (left; ^*α<0.02, ⁺α<0.01). A representative image of the transmigrated SDF-1-pretreated cardiac mesoangioblasts transfected or not with L-selectin is also shown (right). Bar, 30 μm. (b) Human cardiac mesoangioblasts transfected with vectors expressing L-selectin and GFP and pretreated with SDF-1 were injected into the LV chamber of CAL-SCID mice, and after 6 h, the heart and filter organs were collected. The percentage of migrated cells is calculated after performing real-time PCR for GFP with the different samples. A mean of five independent experiments run in triplicate is shown (^*α<0.025, ⁺α<0.015)