Figure 1 | Cell Death & Differentiation

Figure 1

From: SOX2 has a crucial role in the lineage determination and proliferation of mesenchymal stem cells through Dickkopf-1 and c-MYC

Figure 1

Analysis of SOX2 expression and proliferation in hMSCs. (a) RT-PCR of SOX2 in tera-1, hUCB–MSCs (UCB), hAD–MSCs (AD), and hBM–MSCs (BM). SOX2 expression in hUCB–MSCs was lower than in tera-1 cells. (b) Real-time PCR of SOX2 in hMSCs. The expression of SOX2 in hUCB–MSCs was higher than in other hMSCs. (c) Immunocytochemistry of SOX2. SOX2 expression in the nucleus was found in tera-1 cells and hUCB–MSCs. The scale bar represents 10 μm. (d) The hUCB–MSC population decreased after SOX2 knockdown. The scale bar represents 100 μm. (e) SOX2 expression decreased by 10% of the sh-control value after SOX2 knockdown by lentivirus infection. (f) The proportion of cells in S and G0/G1 phase decreased and increased, respectively, after SOX2 knockdown. (g) Cell proliferation significantly decreased after SOX2 knockdown, as indicated by the MTT assays. *P<0.05; **P<0.01; ***P<0.001

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