Figure 3 | Cell Death & Differentiation

Figure 3

From: SOX2 has a crucial role in the lineage determination and proliferation of mesenchymal stem cells through Dickkopf-1 and c-MYC

Figure 3

SOX2 knockdown alter WNT signaling through DKK1. (a and b) PCR array results. The expression of 88 frequently cited stem cell genes were compared between sh-control and sh-SOX2 cells. The expression of AXIN1, KRT15, and GJB2 increased by more than four-fold, and the expression of c-MYC, PARD6A, and CDH1 decreased by more than four-fold after SOX2 knockdown. (c) Real-time PCR analysis of WNT-related candidate genes. The expression of DKK1 was significantly decreased, but the expression of β-CATENIN was increased after SOX2 knockdown. *P<0.05; **P<0.01. (d) The western blot indicates reduced phosphorylated β-CATENIN (p-β-CATENIN) expression and increased total β-CATENIN (total-β-CATENIN) expression after SOX2 knockdown in hUCB–MSCs. Protein levels were normalized to β-ACTIN using Image J analysis software. (e) The TOP/FOP Flash assay shows increased WNT signaling in sh-SOX2 cells. ***P<0.001

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