Table 1 Summary of binding affinity, epitope competition and in vitro cell-killing data for Fas agonists

From: A series of Fas receptor agonist antibodies that demonstrate an inverse correlation between affinity and potency

  

Competition with FasL

Competition with E09 IgG

Jurkat cell killing

Ligand

Fas K d , nM

IC 50 , nM

Max. Inh., %

IC 50 , nM

Max. Inh., %

EC 50 , nM

Efficiency, %

FasL

14.0

ND

ND

ND

ND

7.1

94.0

E09

17.0

1.4

100.0

0.9

100.0

0.9

80.0

DX2

7.3

2.4

98.0

1.6

34.0

1.4

16.0

SM1.1

5.2

5.5

86.0

1.8

100.0

4.0

26.0

  1. The Kd values were determined by SPR analysis of binding to recombinant Fas. Each antibody was also tested for their ability to compete the Fas:FasL and the Fas:E09 interactions in biochemical competition assays to calculate an IC50 value (nM) and maximum inhibition value (%). Cell viability assays were performed on Jurkat cells using a titration of anti-Fas molecules to determine the maximal killing efficiency (%) and EC50 (nM)
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