Table 2 Fas binding kinetics of E09 and variant antibodies

From: A series of Fas receptor agonist antibodies that demonstrate an inverse correlation between affinity and potency

IgG name

Optimisation method

k on , M −1  s −1

k off , s −1

K d , nM

K d gain vs parent

E09

(Parent antibody)

1.9E+05

3.2E-03

17

1

EP12r_E01

Directed Evolution

1.8E+05

1.8E-03

10

2

EP4b_E03

Directed Evolution

2.2E+05

6.4E-04

2.9

6

EP5b_E05

Directed Evolution

3.4E+05

3.1E-04

0.91

19

EP6b_B01

Directed Evolution

6.4E+05

2.3E-04

0.35

49

E09_Y58W

Point Mutation

1.7E+05

1.7E-02

98

0

E09_Y52aR

Point Mutation

1.7E+05

8.2E-03

47

0

E09_R52bS

Point Mutation

1.5E+05

3.5E-04

2.4

7

  1. Values for kon, koff and Kd were calculated following SPR analysis of binding to recombinant Fas. The Kd gain of each variant relative to the parent antibody E09 was also calculated
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