Figure 5
From: Mitochondrial fusion is regulated by Reaper to modulate Drosophila programmed cell death
The GH3 domain of Reaper is required for MFN2 interaction. (a–e) Colocalization of reaper deletion mutants with GFP-MFN2 is shown. Hela cells were cotransfected with GFP-MFN2 and (a) Flag-Reaper, (b) Flag-Reaper 31–65, (c) Flag-Reaper 1–42, (d) Flag-Reaper 39–65 or (e) Flag-Reaper–33 and stained with anti-Flag antibody and an Alexa 594 secondary. (f and g) Cells were transfected with (f) Flag-ReaperΔGH3-ActA or (g) Flag-Reaper-ActA and stained with cytochrome c antibody to visualize mitochondria
