Figure 9
From: Mitochondrial fusion is regulated by Reaper to modulate Drosophila programmed cell death
dMFN inhibits Drosophila apoptosis. (a) S2 cells were transfected with pMT-Reaper-HA and pAC5.1 or pAC5.1-dMFN. Reaper-HA expression was induced for 3 or 6 h with CuSO4. Cell death was analyzed by counting cells with apoptotic morphology. (b) Wing discs were dissected from untreated or irradiated larvae expressing en-gal4 (control) or en-gal4 with UAS-dMFN. Discs were stained with an anti-Engrailed antibody and active caspase 3 antibody. Discs were also dissected from untreated larvae expressing en-gal4 with UAS-dMFN dsRNA and stained for engrailed and active caspase 3. (c) Quantitation of cells with active caspase 3 (CP3+) from B. (d) Wings from adult flies expressing engrailed-gal4 UAS-dMFN-RNAi or driver control
