Figure 1

HIF2α protects CNS neural progenitors from cell death. (a–f) Lateral views of acridine orange (AO) staining of wild-type (WT; a), hif2α translation-blocking MO (hif2α ATG-MO; b), hif2α SPL-MO (c), hif1α ATG-MO (d), hif3α ATG-MO (e) and hif1α, hif3α dual ATG-MO (f) 24 h.p.f. embryos. The apoptotic cells are labeled with white spots. (g–i) Transverse brain sections with TUNEL (bright green) and DAPI (blue) staining in WT (g), hif2α ATG-MO (h) and hif1α, hif3α dual ATG-MO (i) 48 h.p.f. embryos. (j and k) Lateral views of acridine orange (AO) staining of WT (j), hif2α ATG-MO (k) 12 h.p.f. embryos. (l and m) Lateral views of pcna expression in WT (l) and hif2α ATG-MO (m) 12 h.p.f. embryos. (n and o) Apoptosis analysis of p53 ATG-MO (n) and p53, hif2α dual ATG-MO (o) 24 h.p.f. embryos by AO staining. (p and q) Apoptosis analyses of control (p) and hif2α ATG-MO (q) 42 h.p.f. p53^M214K mutant embryos by AO staining. (r and s) Colocalization of TUNEL signaling (in green) and nes expression (in red) in WT (r) and hif2α ATG-MO (s) 56 h.p.f. embryos. nes was stained by fluorescence in situ hybridization. The TUNEL-positive, nes-positive cells (in yellow) represent the apoptotic NPCs. (t) Percentages of normal, moderate and severe apoptosis in the WT control (n=112), hif1α ATG-MO (n=72), hif2α ATG-MO (n=114), hif2α SPL-MO (n=66), hif3α ATG-MO (n=50) and hif1α, hif3α dual ATG-MO (n=137) embryos