Figure 4 | Cell Death & Differentiation

Figure 4

From: Selective regulation of IP3-receptor-mediated Ca2+ signaling and apoptosis by the BH4 domain of Bcl-2 versus Bcl-Xl

Figure 4

Determination of the residues in BH4-Bcl-2 critical for inhibiting IICR. (a) Zoomed picture of the BH4 domain of Bcl-2 showing its surface-accessible residues is depicted. (b) The inhibitory properties of different BH4-Bcl-2 mutant versions (60 μM), in which the surface-accessible residues were altered into alanines, were monitored in unidirectional 45Ca2+-flux assays in permeabilized MEF cells. Four of the six single alanine mutations (K17A, H20A, Y21A and R26A) had the largest effect on the inhibitory action of the wild-type (wt) BH4-Bcl-2 domain

Back to article page