Figure 4
SOCS6-mediated mitochondrial fragmentation is tightly linked to its ability to target to mitochondria. AD293 cells were transfected with pcDNA3 and vectors encoding the full-length and mutant SOCS6 protein. (a) Schematic diagrams of the full-length and mutant SOCS6. The SH2 domain and SOCS6 box are indicated by shaded and hatched boxes, respectively. Mitochondrial localization and distribution pattern of the individual SOCS6 proteins, mitochondrial morphology, and apoptosis are summarized. (b) Immunofluorescence of the full-length and mutant SOCS6 was performed after labeling cells with MitoTracker. Representative confocal images are shown. Scale bar: 10 μm. (c) Cells were scored for SOCS6 localization and mitochondrial morphology, and are summarized in the bar graphs. A total of 300 transfected cells were counted in each condition, and data were derived from three independent experiments. (d) Cells were subjected to the apoptosis assay of sub-G1 apoptotic fractions by FACS analysis. Data were derived from three separate experiments and are presented as the means±S.E.M.
