Figure 2
ECMPs improve efficiency of hESC differentiation to DE, PGT, PF endoderm, and PE. HESCs were cultured on MGEL and FN and VTN (FN+VTN) using previously published protocols.2, 8, 9 (a) Representative images of αSOX17 immunofluorescence of HUES9 hESCs differentiated to DE on MGEL and FN+VTN (mean±S.E.M.). (b) Quantification of HUES9 hESCs stained by SOX17 cells out of total cell number (n=3; mean±S.E.M.). (c) Flow cytometric analysis of CXCR4 expression of HUES9 hESCs differentiated to DE on MGEL and FN and VTN (FN+VTN). Gene expression analysis for markers of (d) DE (SOX17, FOXA2, CXCR4), (e) PGT (HNF1β, HNF4α), and (f and g) PF and PE (HNF6, PDX1) of HUES9 hESCs differentiated to DE, PGT, PF, and PE on MGEL and FN+VTN. (n=3; error bars, S.E.M.). Asterisks indicate statistical significance relative to MGEL as determined by a two tail t-test
