Figure 2

Analysis of CDKs, cyclins and endogenous CDK inhibitors in human neutrophils. Expression levels of human neutrophil CDKs (a), cyclins (b) and endogenous CDK inhibitors (c) as analysed by Illumina Gene Chip. Neutrophils isolated from four independent donors (n=4) and treated for 4 h with media alone (Control), R-roscovitine (20 μM), LPS (100 ng/ml) or a combination before RNA isolation and interrogation by microarray. Result shows gene expression levels (fluorescence units, f.u.) as mean±S.E.M. Significant differences P<0.001 between Control and R-roscovitine are shown as *, between LPS and R-roscovitine/LPS as #, and between LPS and control by +, analysed using ANOVA with post hoc multivariate analysis by Student’s Newman–Keuls