Figure 4 | Cell Death & Differentiation

Figure 4

From: Phylogenetic distinction of iNOS and IDO function in mesenchymal stem cell-mediated immunosuppression in mammalian species

Figure 4

BM-MSCs isolated from different species have the same immunosuppression effects on Spls or PBMCs. PBMCs or Spls stained with CFSE were cocultured with BM-MSCs (2.0 × 104 cells per well in 96-well plate) at a ratio of 1 : 10 (MSC: T cell) for 96 h, in the presence of conconavalin A (Con A) (1 μg/ml; for rat, rabbit, hamster, and pig), or anti-CD3 (1 μg/ml; for human, monkey, and mouse). CFSE fluorescence intensity reduction of PBMCs or Spls was detected by flow cytometry. Data are representative of three independent experiments

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