Figure 1

Loss of Bak exacerbates lymphocytosis in BCL-2tg mice. Enumeration of total leukocytes and indicated lymphoid populations in the (a) spleen and (b) thymus of 12- to 14-week-old male mice (n=6–10 per genotype: WT, white; Bak^−/−, light grey; BCL-2tg, dark grey; Bak^−/−BCL-2tg, black). B220⁺IgM/D⁺ indicates B220⁺ cells that are IgM⁺ and/or IgD⁺. Bars represent mean±S.E.M.; see also Supplementary Table 2. Statistical significance is shown only for BCL-2tg versus Bak^−/−BCL-2tg; *P<0.05, **P<0.01, Mann–Whitney test. (c) Expression of the indicated Bcl-2 family proteins in sorted DP thymocytes determined by western blot analysis of cells from two independent mice for each genotype