Figure 6
From: Inhibition of autophagy, lysosome and VCP function impairs stress granule assembly
Chemical inhibition of lysosomes or genetic perturbation of autophagy leads to formation of SGs that contain RPL19. (a, b) HeLa cells were treated for 45 min with arsenite (Ars.) alone or following ammonium chloride (NH4Cl) pre-treatment. Cells were fixed and labeled with anti-TIA-1, RPL19 and DAPI. (b) Quantitation of colocalization of RPL19 and TIA-1 is shown. Error bar, S.E.M. **P<0.01. (c, d) Cells treated as described in a were fixed, subjected to in situ hybridization with a 28S rRNA probe coupled to Alexa594 and labeled with anti-G3BP and DAPI. (d) Quantitation of the % of TIA-1-positive SGs located outside or inside the 28S rRNA perinuclear-enriched region is shown. Error bar, S.E.M. *P<0.05. (e, f) Atg16+/+, Atg5−/− and Atg16−/− MEFs were treated for 45 min with Ars. and labeled with anti-TIA-1, RPL19 and DAPI. (f) Quantitation of colocalization of RPL19 and TIA-1 is shown. Error bar, S.E.M. **P<0.01. (a–c) 2.5 × magnification of the selected area. See also Supplementary Figure S6
