Figure 1 | Cell Death & Differentiation

Figure 1

From: β-Catenin and NF-κB co-activation triggered by TLR3 stimulation facilitates stem cell-like phenotypes in breast cancer

Figure 1

TLR3 but not TLR5, 7, and 8 activation associates with breast CSC-like properties.(a) SUM190 cells treated with 1 μg/ml of poly(I:C) for different days show a morphological change with the formation of sphere-like live aggregates. Scale bars, 100 μm. The fractions of viable cells after poly(I:C) treatment for different days were determined by trypan-blue exclusion assay. Bars denote the standard error for proportions (n=3). (b) Trend changes of stemness genes at days 1, 2, 4, and 8 in poly(I:C)-treated SUM190 cells from three independent experiments. Error bars indicate S.D. (c) Western blot analysis of transcriptional factors in SUM190 cells treated with vehicle control or poly(I:C). α-tubulin and Lamin A/C: internal loading controls for cytoplasmic (Cyto) and nuclear (Nu) proteins. (d) Quantitative real-time PCR analysis of indicated genes in SUM190, SUM149, BT-483, and Cama-1 cells after treatment with 1 μg/ml of poly(I:C) for 4 days. GAPDH mRNA was used to normalize variability in template loading. Data represent the average±S.D., n=3; *P<0.05

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