Figure 4 | Cell Death & Differentiation

Figure 4

From: Dysregulation of Wnt inhibitory factor 1 (Wif1) expression resulted in aberrant Wnt-β-catenin signaling and cell death of the cloaca endoderm, and anorectal malformations

Figure 4

In vivo and in vitro deletion of Shh elevated Wif1 expression. Wif1 was weakly expressed at E11.5 in control littermates (a). Deletion of Shh induced precocious Wif1 expression in the cloaca membrane endoderm and cloaca canal endoderm at E11.5 (b). By E12.5, a similar expression pattern of Wif1 was observed in both control (c) and Shh mutant embryos (d). However, the cm in Shh mutant was much thinner than the control embryo. Both cytoplasmic and nuclear β-catenin immunoreactivity decreased markedly in Shh mutant embryo (f) as compared with the control littermate (e). In vitro inhibition of Shh signaling by cyclopamine induced ectopic expression of Wif1 in the entire cloaca endoderm and surface ectoderm of the GT explant (h; n=5) compared with the control (g). Scale bar: 50 μm

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