Figure 1 | Cell Death & Differentiation

Figure 1

From: Disruption of the temporally regulated cloaca endodermal β-catenin signaling causes anorectal malformations

Figure 1

Temporally labeled Shh-expressing URS endodermal cells contribute to the ectodermal epithelia. The expression of the Shh gene in sagittal sections of wild-type embryos at E10.5, E11.5, E12.5 and E13.5 (a–d). The asterisk in (a) indicates future URS derived from the proximal umbilical mesenchyme. Ventral view of the genital tubercle (e–j). Tissue labeling experiments of Shh-expressing cells were performed at E15.5. ShhCreERT2/+; R26LacZ/+ embryos subsequent to TM administration at E8.5, E9.5, E10.5, E11.5, E12.5 or E13.5 (e–j). The red arrows indicate the LacZ-positive ectodermal cells. β-Catenin was expressed in the endoderm, including the URS epithelia, at E11.5 in the wild-type embryos (k). b, Bladder; c, cloaca; gt, genital tubercle; hl, hindlimb; r, rectum; t, tail; uc, umbilical cord; urs, urorectal septum

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