Figure 4

Time-course expression analysis of TUNEL and cleaved caspase-3 in the β-catenin GOF mutants. Sagittal sections of the embryos at E11.5, E12.5, E13.5, E14.5 and E15.5 subsequent to E9.5-TM treatment (a–t). The number of apoptotic cells in the URS epithelia of the β-catenin GOF mutants was less than that observed in the controls at E11.5 and E12.5 (a, b, f, g, k, l, p and q; red arrows and surrounded by red dotted line). The number of apoptotic cells was increased in the mutant URS epithelia at E13.5, and such cells were also observed in the mutant URS at E14.5 and E15.5 (c–e, h–j, m–o and r–t; blue arrows and surrounded by blue dotted line). urs, urorectal septum