Figure 1 | Cell Death & Differentiation

Figure 1

From: Small GTPase CDC-42 promotes apoptotic cell corpse clearance in response to PAT-2 and CED-1 in C. elegans

Figure 1

CDC-42 is a candidate SRGP-1 target during engulfment signaling. (a) Knockdown of cdc-42 and loss of srgp-1(ok300) have opposite effects on cell corpse clearance in C. elegans. Staged animals (P0) were grown on RNAi plates and freshly hatched L1 progeny larvae (F1) were scored for corpse numbers in the head region. All strains carry the ced-5(tm1949) mutation in the background. Data are shown as average±S.D. (n≥25). rho-1(RNAi) causes embryonic lethality and thus is not done (n.d.). (b) The SRGP-1 GAP domain binds GTP-bound CDC-42 in vitro. The GTP- and GDP-bound isoforms of 6xHis-tagged CDC-42 and RHO-1 (CDC-42: Q61L and T17N, RHO-1: Q63L and T19N, respectively) were used for pulldowns. GST-fusions used: GST (alone), the GAP domain of SRGP-1, the GTP hydrolysis-deficient SRGP-1 GAP (R563A) domain22 and the GAP domain of RGA-3, a potential GAP of both RHO-1 and CDC-42 (see Schonegg and Hyman26)

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