Figure 5 | Cell Death & Differentiation

Figure 5

From: Erratum: Obatoclax (GX15-070) triggers necroptosis by promoting the assembly of the necrosome on autophagosomal membranes

Figure 5

GX15-070 triggers the assembly of the necrosome on autophagosomes. (a) TE671 and RMS13 cells were treated with 200 nM GX15-070 for the indicated time points. RIP1 was immunoprecipitated (IP) using anti-RIP1 antibody. Atg5, FADD, RIP3 and caspase-8 were detected by western blotting. (b) TE671 and RMS13 cells were transduced with control vector (shCtrl) or vectors containing two distinct shRNA sequences against Atg5 (shAtg5#1, shAtg5#2) and treated with 100 nM GX15-070 for 48 h. RIP1 was immunoprecipitated (IP) using anti-RIP1 antibody. Atg5, FADD, RIP3 and caspase-8 were detected by western blotting

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