Figure 2

Depletion of IRF4 in neurons led to potentiated cerebral injury. (a–c) The mice were subjected to I/R for 24 or 72 h. (a) 2,3,5-Triphenyl-2H-tetrazolium chloride (TTC)-stained brains from WT and IRF4-KO mice at the indicated times after I/R. Scale bar: 10 mm. (b) Quantification of infarct volumes 24 and 72 h after I/R. Four to eight independent experiments were performed for each time point. (c) The mice were assessed for neurological deficit scores at the indicated time points. Four to eight independent experiments were performed for each time point. (d) Representative MRI images of live mice 24 h after I/R. Scale bar: 5 mm. (e) Quantification of infarct volumes using MRI. Four to five independent experiments were performed. *P=0.0046 (b, 24 h), 0.0460 (b, 72 h), 0.0032 (c, 24 h), 0.0012 (c, 72 h) and 0.0272 (e) compared with WT mice unpaired Student’s t-test. The values are the means±S.E