Figure 6

Enforced re-expression of the CB₁ receptor rescues HD-like molecular-pathology markers in the striata of R6/2 mice. R6/2 mice (3.5–4 weeks old) and WT littermates were injected stereotactically into the dorsolateral striatum with a recombinant adeno-associated virus (AAV) encoding HA-tagged CB₁ receptor or the empty vector as control (n=10–12 animals per group). At week 8 of age, animals were killed for histological analyses in the striatum. (a) DARPP-32 immunoreactivity (relative values of DARPP-32-positive cells/total cell nuclei). Representative images are shown. (b) GAD-67 immunoreactivity (relative values of GAD-67-positive area/total cell nuclei). (c) PSD-95 immunoreactivity (relative values of PSD-95-positive area/total cell nuclei). (d) Phospho-S6 immunoreactivity (relative values of pS6-positive cells/total cell nuclei). Data were analyzed using unpaired Student’s t-test. *P<0.05 and **P<0.01 from the corresponding WT-empty group; ^#P<0.05 and ^##P<0.01 from the corresponding R6/2-empty group