Figure 1 | Cell Death & Differentiation

Figure 1

From: Repression of p53-target gene Bbc3/PUMA by MYSM1 is essential for the survival of hematopoietic multipotent progenitors and contributes to stem cell maintenance

Figure 1

Mysm1 deficiency results in dysregulation of p53-target gene expression in HSPCs. qPCR analysis of Lin−cKit+Sca1+ cells sorted from the bone marrow of CreERT2-transgenic Mysm1flox/flox mice following tamoxifen-induced Mysm1 gene inactivation (black bars). CreERT2 Mysm1+/flox mice that received the same tamoxifen treatment (gray bars), and sham-injected Mysm1flox/flox mice (white bars) were used as controls. Transcript levels of (a) Mysm1 and p53, (b) classical p53 stress-response genes, (c) regulators of HSC quiescence, and (d) HSC-specific p53 target genes are presented. All gene expressions were normalized against Hprt expression. Bars represent mean±S.E.M.; *P<0.05, **P<0.01, ***P<0.001, NS, nonsignificant using Student’s t-test; data from ≥5 mice per group

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