Figure 6

Partial rescue of hematopoietic stem and progenitor cell functions in Mysm1^−/−Puma^−/− mice. Competitive bone marrow transplantations were set up; B6.SJL-CD45.1 bone marrow was mixed with CD45.2 bone marrow from wild type, Puma^−/−, Mysm1^−/−, or Mysm1^−/−Puma^−/− mice, and was transplanted into four independent groups of lethally irradiated recipients. Recipients were analyzed for the relative contribution of CD45.2 donor cells to hematopoiesis at 20 weeks after reconstitution. (a) Percentage contribution of wild-type, Puma^−/−, Mysm1^−/−, or Mysm1^−/−Puma^−/− donors to Lin⁻cKit⁺Sca1⁺ (KLS) cells, Flt3⁻ KLS HSCs, and Flt3⁺ KLS MPP cells. (b) Flow cytometry histograms of the bone marrow of the chimeric mice gated on Lin⁻cKit⁺Sca1⁺ cells (KLS, left), Flt3⁻ KLS HSCs (middle), or Flt3⁺ KLS MPP cells (right). Histogram gates and percentages indicate CD45.2⁺CD45.1⁻ donor cells. (c–e) Percentage contribution of wild type, Puma^−/−, Mysm1^−/−, or Mysm1^−/−Puma^−/− donors to (c) CMPs (Lin⁻cKit⁺Sca1⁻IL7R⁻CD34⁺CD16/32⁻), granulocyte-macrophage progenitors (GMP, Lin⁻cKit⁺Sca1⁻IL7R⁻CD34⁺CD16/32⁺), and CD11b⁺ myeloid lineage cells in the bone marrow and spleen; (d) CLPs (Lin⁻cKit⁺Sca1⁺IL7R⁺), splenic B cell (B220⁺), splenic CD4 T cell, and splenic CD8 T-cell populations; (e) NK cell progenitors (NKPs, Lin⁻CD122⁺NK1.1⁻CD11b⁻), immature bone marrow NK cells (iNKs, Lin⁻CD122⁺NK1.1⁺CD11b⁻), and mature NK cells in the bone marrow (Lin⁻CD122⁺NK1.1⁺CD11b⁺) and spleen (CD3⁻NK1.1⁺). Bars show means±S.E.M.; *P<0.05, **P<0.01, ***P<0.001, NS, nonsignificant using ANOVA with Bonferroni post-hoc test