Figure 1 | Cell Death & Differentiation

Figure 1

From: PUMA promotes apoptosis of hematopoietic progenitors driving leukemic progression in a mouse model of myelodysplasia

Figure 1

Stem/progenitor cell characterization in NHD13 mice. (a) Flow-cytometric analysis of LK and LSK cell populations gated on lineage-negative cells; and SLAM populations as a subset of LSK cells. Cell populations examined include LT-HSCs (CD150+CD48−), ST-HSCs (CD150−CD48−) and MPPs (CD150−CD48+). Numbers depict percentages of total nucleated cells. Plots shown are representative of three individual experiments. (b) Absolute quantification of individual SLAM cell populations (per femur and tibia of each mouse) (n=9 mice per genotype). (c) Apoptosis analysis of LSK and MPP cell populations from WT (n=14) and NHD13 (n=15) mice. Results were normalized to caspase-3/7 activation levels in WT cells. (d) Schema for chimeric transplant experiment. Four recipient mice were used for each population of cells. Total number of recipient mice=24. (e) Chimerism results for transplanted mice. Results are derived from analyses of monthly bleeds for 4 months in total (except for LK cell transplants) and represent percentage of peripheral blood cells that were donor-derived. **P<0.01; ***P<0.001; ****P<0.0001

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