Figure 7

The differing roles of p53 and PUMA in progression of MDS to AML. (a) Cell-cycle analysis of LSK cells based on DAPI and Ki67 staining. Data show percentage of total cells in various stages of cell cycle. (b) DNA damage analysis with data normalized to the levels seen in wild-type cells for Puma-deficient mice. γH2AX staining is expressed as a percentage of activated caspase-3/7 negative, G0 (non-cycling) cells (n=9 per genotype); Histogram to the right shows a single graphical representation of the data. (c) DNA damage analysis with data normalized to the levels seen in wild-type cells for WT (n=9), NHD13 (n=8), p53 KO (n=4) and NHD13;p53 KO (n=5) mice; Histogram to the right shows a single representation of the data. (d) mRNA expression of genes involved in the NHEJ DNA damage response. Results have been normalized to HPRT and actin levels (n=3 per genotype); T/+, transgenic; *P<0.05; **P<0.01