Figure 2 | Cell Death & Differentiation

Figure 2

From: Functional disparities among BCL-2 members in tonsillar and leukemic B-cell subsets assessed by BH3-mimetic profiling

Figure 2

Differential protein expression of BCL-2 family members in primary human B cells. (a) Western blot analysis for indicated BCL-2 family members in tonsil B cells, fluorescence-activated cell sorting (FACS)-purified as shown in Figure 1a. Data is representative of two (BFL-1, BIK and BIM) to four (BCL-2, MCL-1 and BCL-XL) independent experiments. (b) Quantified western blots shown in a corrected for expression of Actin and relative to naive B cells. BFL-1 expression could not be quantified due to the low expression level. Data are average values of three to four different experiments with S.E.M. (c) Fluorescence microscopy image of a tonsil section showing MCL-1 protein staining in a CD20+ GC in the tonsil. Scale bar, 100 μm in overview image (left panel) and 20 μm in selection (right panel). Images are representative of three individual experiments. Statistics were calculated in relation to Bn cells. *P⩽0.05, **P⩽0.01

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