Figure 4

MCL-1 associates with BIM and BCL-XL associates with BIK in tonsil B cells. (a) Western blots after IP experiments in total B cells purified from the tonsil. Shown are the indicated proteins in the lysate after IP (cleared lysate) or the indicated proteins that were pulled down (IP). Background bands in the IP fraction were visualized by incubation with only the labeled secondary antibody and are indicated by (*). The experiment shown is representative of four individual experiments. (b) IP as in (a), but either with or without treatment of 30 μM A-1210477 for 4 h at 37 ºC. Experiment is representative of two individual experiments. (c) IP as in (a), but either with or without treatment of 10 μM WEHI-539 for 4 h at 37 °C. Experiment is representative of two individual experiments