Figure 4

Signal transduction in response to BMP2 and BMP4 is affected by long-term exposure to BPA and B(a)P. (a) Western blot bright-field images of MCF10A cells exposed 60 days to BPA or B(a)P (10⁻¹⁰ M) and treated for 4 days with BMP2 or BMP4 (15 ng/ml). UT are the BMP-untreated cells. Data are representative of four independents experiments. (b) Column scatter plot showing the quantification of BMP receptors 1a (left panel) or 1b (right panel) detected by western blot normalized against the level of tubulin. BPA or B(a)P-exposed cell lines were treated for 4 days with BMP2 (15 ng/ml). Data are represented as mean +/− S.E.M., n=4. (c) Column scatter plot showing the percentage of BMP receptors 1a- (left panel) or 1b-positive MCF10A cells (right panel) exposed 60 days to BPA or B(a)P (10⁻¹⁰ M) and treated for 4 days with BMP2 (15 ng/ml) by flow cytometry. Data are represented as mean +/− S.E.M., n=11. (d) Column scatter plot showing the quantification of BMP receptors 1a (left panel) or 1b (right panel) detected by western blot normalized against the level of tubulin. BPA or B(a)P-exposed cell lines were treated for 4 days with BMP4 (15 ng/ml). Data are represented as mean +/− S.E.M., n=4. (e) Column scatter plot showing the percentage of BMP receptors 1a- (left panel) or 1b-positive MCF10A cells (right panel) exposed 60 days to BPA or B(a)P (10⁻¹⁰ M) and treated for 4 days with BMP4 (15 ng/ml) by flow cytometry. Data are represented as mean +/− S.E.M., n=11