Figure 5
Serum Ig levels are elevated in Mcl-1tg/lpr mice. (a) ELISA quantification of Ig levels (IgA, IgG1, IgG2a, IgG2b, IgG3 and IgM) in sera of 14-week healthy WT (white, n=5), Mcl-1tg (light grey, n=4), lpr (dark grey, n=6) and Mcl-1tg/lpr (black, n=6) mice. Bars represent mean±S.E.M. (b) ELISPOT enumeration of the total number of antibody-secreting cells (ASCs) in the spleen and bone marrow (per femur) of healthy 14-week WT (white, n=7), Mcl-1tg (light grey, n=6), lpr (dark grey, n=6) and Mcl-1tg/lpr (black, n=5) mice. Data points represent individual mice with mean±S.E.M indicated. (c) Representative confocal images of snap frozen kidney sections stained with FITC-labelled antibody for the presence of IgG-, IgM- or IgA-deposition (green) in the glomeruli; nuclei are stained with DAPI (blue). One mouse of each genotype is shown. White arrows indicate Ig deposition on the glomerular capillary loops. Scale bar=25 μm. (d) Enumeration of staining patterns observed in snap frozen kidney sections analyzed for the presence of IgG-, IgM- or IgA- deposition (see also Supplementary Figure S6). Graphs indicate the proportions of WT (n=2), Mcl-1tg (n=2), lpr (n=8) and Mcl-1tg/lpr (n=4) kidneys showing no staining of glomeruli (white), staining of Ig deposition on mesangial cells only (grey), or staining of Ig deposition on both glomerular capillary tubules and mesangial cells (black). *P<0.05, **P<0.01, ***P<0.001, calculated by Student’s T-test with Welch’s correction
