Figure 6
ANA levels are elevated in Mcl-1tg/lpr mice. (a) ELISA quantification of dsDNA- and ssDNA-specific IgG (top panels) and dsDNA- and ssDNA-specific IgM (bottom panels) (arbitrary units/ml) in sera of sick lpr (checkered dark grey, n=13) and Mcl-1tg/lpr (checkered black, n=13) mice and healthy 21-week WT (white, n=7), Mcl-1tg (light grey, n=9), lpr (dark grey, n=9) and Mcl-1tg/lpr (black, n=7) mice. (b) Representative confocal images of Hep-2 cells stained with sera from sick lpr mice or Mcl-1tg/lpr mice. Staining intensity scoring (0 to 3+) and type of staining (N=nuclear, P=peripheral) are indicated. Positive control=sera from aged Fasgld/gld mouse lacking functional FasL; negative control=sera from 8-week healthy WT mouse. (c) ANA fluorescence intensity of Hep-2 cells, measured in arbitrary units, from sera of sick lpr (grey, n=12) and Mcl-1tg/lpr (black, n=11) mice. Data represent mean±S.E.M. *P<0.05, **P<0.01, ***P<0.001 calculated by Student’s T-test with Welch’s correction. (d) Patterns of staining: homogenous nuclear staining (green); staining peripheral to the nucleus (white); or no detectable staining (black) for the mice analyzed in (c)
