Figure 2

Bone resorption is enhanced in Men1^Runx2Cre mice, caused by Men1 deficiency in osteocytes. (a and b) Osteoclast numbers per bone perimeter (N.Oc/B.Pm) (a) and osteoclast surface per bone surface (Oc.S/BS) (b) in sections of femoral trabecular bone were measured by histomorphometry (n=4 or 5). (c) Determination of resorption by assessment of the serum level of the biomarker CTX in 12-week-old female Men1^flox and Men1^Runx2Cre mice (n=5). (d) Number of multinucleated TRAP-positive osteoclasts was counted from primary co-cultures of wild-type BMCs with Men1^flox or Men1^{gtRosaCreERT2} primary osteoblasts (n=3). (e–g) Osteoclastogenesis of primary co-cultures of wild-type BMCs with Men1^flox or Men1^{gtRosaCreERT2} primary osteocytes was visualized by TRAP staining (e). Number of multinucleated TRAP-positive cells (f) and their area (g) were determined (n=3). *P<0.05, **P<0.01. Data are represented as mean±S.E.M. Scale bar: 25 μm