Figure 1
From: Immunoproteasome dysfunction augments alternative polarization of alveolar macrophages
Expression of immunoproteasome subunits LMP2 and LMP7 is induced in M1- and M2-polarized alveolar macrophages. (a) Immunoproteasome subunit gene expression in MH-S cells or primary alveolar macrophages from BALB/c or C57BL/6 polarized into M1 or M2 for 24 h by stimulation with LPS/IFNγ or IL-4, respectively. Non-polarized cells (M0) served as controls: Psmb9 (LMP2), Psmb10 (MECL-1) and Psmb8 (LMP7) RNA expression is displayed relative to Actb. Results are combined data from at least three independent experiments (mean+S.E.M.). (b) Immunoproteasome subunit LMP2 and LMP7 protein expression as well as 20S α1–7 subunits in primary alveolar macrophages from C57BL/6 mice polarized for 24 h. Results are representative for three independent experiments, densitometric analysis shows the fold-increase over M0 in three different experiments (mean+S.E.M.). (c) Proteasome activity in polarized alveolar macrophages from C57BL/6 mice detected after 48 h stimulation by ABPs MV151 (labeling all catalytically active β-subunits), LW124 (β1 and LMP2) or MVB127 (β5 and LMP7). Densitometric analysis displays the combined data from three individual experiments (mean+S.E.M.) loaded on the same gel. Statistical analysis (a)–(c): One-way ANOVA with Dunnetts’s multiple comparison test (all columns versus control column (M0), *P<0.05, **P<0.01, ***P<0.001)
