Figure 3
From: Immunoproteasome dysfunction augments alternative polarization of alveolar macrophages
LMP7 deficiency affects M2 polarization at the transcriptome level. (a) Heatmap depicting genes that are significantly upregulated in LMP7−/− M2 compared with wt M2 (green, least expression; red, highest expression). (b) qPCR analysis of selected new M2 marker genes from the heat map: Rnase2a, Cish, Clec10a, Tfrc, Ptgs1, Chchd10, Sema4b, Il1r1 and Ccr5 are shown relative to Actb. Results are the mean+S.E.M. from three individual C57BL/6 wt or LMP7−/− mice. (c) GO analysis: top GO terms enriched among genes that are upregulated in LMP7−/− M2, relative to wt M2. One-way ANOVA with Bonferroni’s multiple comparison test (***P<0.001)
