Figure 4

LMP7 deficiency augments alveolar macrophage M2 signaling. (a) Enrichment of IL-4 signaling according to Broad Institute (http://www.broadinstitute.org/) 'LU_IL4 signaling' related genes in LMP7^−/− M2 compared with wt M2 as shown by GSEA, IL-4 signaling. (b) Volcano plot showing genome-wide changes of gene expression in LMP7^−/− M2 relative to wt M2. X axis represents the average log2 fold change, whereas y axis indicates the -log10P. Significantly changed genes (P<0.05) with × >0.5 are represented by red circles and × <0.5 by blue circles. (c) Irf4 gene expression in primary alveolar macrophages from C57BL/6 wt or LMP7^−/− mice treated with IL-4 for up to 72 h. Results are combined data from three experiments (mean+S.E.M., two-way ANOVA with Bonferroni’s post test, **P<0.01, ***P<0.001). (d) Time course of IRF4 (e) STAT6 (pTyr⁶⁴¹) and AKT (pSer⁴⁷³) pathway activation and expression within 0–180 min after IL-4 treatment in primary alveolar macrophages from C57BL/6 wt or LMP7^−/− mice. Results are representative for two independent experiments. (f) Densitometric analysis of protein levels of IRF4, p-STAT6 and p-AKT relative to β-actin for blots shown in (d) and (e)