Figure 5
From: Immunoproteasome dysfunction augments alternative polarization of alveolar macrophages
IL-13 treatment increases M2 polarization in LMP7−/− alveolar macrophages. (a) LMP2 and LMP7 protein expression in primary alveolar macrophages from C57BL/6 mice polarized for 24 h with IL-13. (b) STAT6 phosphorylation (pTyr641), total STAT6 expression and IRF4 expression up to 72 h after IL-13 treatment, β-actin expression served as loading control (* depicts residual IRF4 signal from first exposure). Results are representative for two independent experiments. (c) M2 marker gene expression Arg1, Ccl17, Retnla and Mrc1 in primary alveolar macrophages from three individual C57BL/6 wt or LMP7−/− mice (mean+S.E.M.). One-way ANOVA with Bonferroni’s multiple comparison test; (**P<0.01, n.s., not significant)
