Figure 6
From: Immunoproteasome dysfunction augments alternative polarization of alveolar macrophages
Effect of LMP7 deficiency on IL4Rα expression in alveolar macrophages. (a) Il4ra mRNA expression analysis of primary alveolar macrophages from C57BL/6 wt or LMP7−/− mice polarized for 24 h with LPS/IFNγ (M1) or IL-4 (M2), relative to Actb expression. Results are combined data from three independent experiments (mean+S.E.M., one-way ANOVA with Bonferroni’s multiple comparison test; n.s., not significant). (b) IL4Rα expression within 0–180 min after IL-4 treatment in primary alveolar macrophages from C57BL/6 wt or LMP7−/− mice and densitometric analysis (the same membrane and thus β-Actin loading control was used for Figure 4d)
