Figure 2

Drug resistance and tumour grade are associated with cell metabolism in OC. (a) BEC index analysis on PEA1 and PEA2 cells upon TRAP1 silencing. Where indicated, cells were transfected with TRAP1-directed or control siRNAs; 72 h after transfection, cells were harvested and total protein lysates were immunoblotted with anti-βF1ATPase, anti-HSP60 and anti-GAPDH antibodies. Immunoreactive bands were quantified by using ImageJ and BEC index was calculated by the formula F1ATPase/HSP60/GAPDH (see Material and Methods section for details). Data are expressed as mean±S.E.M. from five independent experiments. Numbers above bars indicate the statistical significance (P-value), based on the two-tailed Student’s t-test. (b) Total lysates obtained by a set of OC tissues (n=47) were analyzed by WB analysis for BEC index calculation as described in panel (a). The resulting BEC index values were normalized on a reference sample and correlated to clinical parameters of each sample. (c) Kaplan–Meier estimates of the impact of TRAP1 on Overall Survival (P-values determined by the log-rank test)