Figure 3

Modulation of TRAP1 expression causes metabolic shift in OC cells. (a and b) Kinetic profile of ECAR and OCR in PEA1 and PEA2 cells was measured in real-time, under basal conditions and in response to glucose, oligomycin and 2-DG for ECAR (panel a), and in response to mitochondrial drugs oligomycin, FCCP, Antimycin A and Rotenone for OCR (panel b). Indices of glycolytic pathway activation, calculated from PEA1 and PEA2 cells ECAR profile: basal ECAR, maximal ECAR and glycolytic capacity (see also Materials and Methods section). Indices of mitochondrial respiratory function, calculated from PEA1 and PEA2 cells OCR profile: basal OCR, ATP-linked OCR and maximal OCR (see also Materials and Methods section). For each profile is shown one representative out of three independent experiments. Data are expressed as mean±S.E.M. of three measurements, each of them in triplicates. (c and d) Kinetic profile of ECAR and OCR in PEA1 cells transfected with non-targeted control siRNA or TRAP1-directed siRNA was measured as described for PEA1 and PEA2 cells. For each profile, one representative out of three independent experiments is shown. Data are expressed as mean±S.E.M. of three measurements, each of them in triplicates. (Statistical analysis by two-tailed Mann–Whitney test)