Figure 1 | Cell Death & Differentiation

Figure 1

From: Reactivation of mutant p53 by a dietary-related compound phenethyl isothiocyanate inhibits tumor growth

Figure 1

PEITC inhibits cell proliferation and induces apoptosis in a p53R175 mutant-dependent manner. (a) Human tumor cells lines with hotspot p53 mutations and p53 WT were treated with DMSO (control) or PEITC for 3 days. (b) SK-BR-3 and A549 cells transfected with siRNA were treated with DMSO or PEITC for 3 days. Percentage of cell proliferation was determined by the WST-1 assay. (c) Effect of PEITC on apoptosis. Untransfected (cells) or siRNA-transfected SK-BR-3 and A549 cells were treated with DMSO or 4 μM PEITC for 3 days. Apoptosis was measured by Annexin-V staining using a BD LSRFORTESSA instrument. (d) The H1299 cells transfected with pcDNA3, pcDNA3-p53R175, pcDNA3-p53R273 or pcDNA3-wtp53 were treated with DMSO or PEITC for 3 days. Percentage of cell proliferation was determined by the WST-1assay. (e) Effect of PEITC on apoptosis. The H1299 cells transfected with pcDNA3, pcDNA3-p53R175, pcDNA3-p53R273 or pcDNA3-wtp53 were treated with DMSO or 8 μM PEITC for 3 days. Apoptosis was measured by Annexin-V staining using a BD LSRFORTESSA instrument

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