Figure 4
Proteasome degradation of p53 protein upon PEITC treatment in SK-BR-3 and A549 cells. (a) SK-BR-3 cells were treated with the indicated concentrations of PEITC and inhibitor (10 μM Nutlin-3 or 20 μM MG132) for 4 h. (b) SK-BR-3 cells were treated with PEITC (4 or 8 μM), 20 μM MG132 or both for 4 h. (c) SK-BR-3 cells were treated with PEITC (4 or 8 μM), 10 μM Nutlin-3 or both for 4 h. (d) A549 cells were treated with PEITC (4 or 8 μM), inhibitor (10 μM Nutlin-3 or 20 μM MG132) or both for 4 h. Cells were harvested and lysates were prepared. Lysate fractions were resolved by SDS-PAGE and probed with p53 DO-1 antibody. (e) SK-BR-3 cells were treated with the indicated concentrations of PEITC or DMSO for 4 h. Cells were harvested and soluble and insoluble fractions were prepared. Thirty μg of the soluble and insoluble lysate fractions were resolved by SDS-PAGE and probed with p53 DO-1 antibody
