Figure 1
miR-17-92 cluster is downregulated during skeletal muscle differentiation, regeneration and development. (a) Workflow of the identification of myogenesis-associated miRNAs. (b) Volcano plot showing the differentially expressed miRNAs in differentiated C2C12 myotubes compared with myoblasts. The negative Log2-adjusted P-values (y-axis) are plotted against the Log2 fold changes in expression (x-axis). The horizontal dashed line indicates the threshold for significance (P <0.05) and the vertical dashed line indicates the upregulated (right side) and downregulated (left side) miRNAs. miR-17, -20a and -92a are indicated. (c) miR-17-92 is downregulated during myogenesis. C2C12 mouse myoblasts were cultured in growth medium (GM) and then switched to differentiation medium (DM) for 1–7 days. Quantitative RT-PCR was performed to analyze the expression level of miR-17, -18a, -19a, -20a and -92a. The data were normalized to SNORNA234. miR-17-92 expression in myoblasts cultured in DM was set to 1.0. The error bars depict the means±S.D. of three independent cell samples. (d) miR-17-92 is upregulated in skeletal muscle on days 1–3 and downregulated on days 5–10 post-CTX injury. miR-17-92 expression in skeletal muscle before the CTX injection was set to 1.0. The error bars depict the means±S.D. of the samples with six mice in each group. The rest is as in c. (e) miR-17-92 expression in porcine skeletal muscle during post-natal development. Quantitative RT-PCR validated the level of miR-17-92 in porcine longissimus dorsi muscles on postnatal days 35, 63, 98 and 161. The Data were normalized to SNORAN202. miR-17-92 expression on postnatal day 35 was set to 1.0. The error bars depict the means±S.D. of the samples with six pigs in each group. (f) miR-17-92 expression in mouse skeletal muscle during postnatal development. qRT-PCR validated the level of miR-17-92 in mouse hind legs on days 2, 14, 28 and 42. miR-17-92 expression on day 2 was set to 1.0. The rest is as in d
