Figure 6
E2F1 increases the transcription of miR-17-92 in myoblasts. (a–c) E2F1 protein expression during C2C12 myoblast myogenesis (a), mouse muscle regeneration (b) and postnatal growth (c). Beta-actin and beta-tubulin served as the loading controls. (d) Overexpression of miR-17, -20a or -92a reduces E2F1 protein expression in C2C12 myoblasts transfected with the miR-17, -20a or -92a mimics. The data are presented as the means of the samples from three different cell samples. M1, mouse 1; M2, mouse 2. Beta-actin served as the loading control
