Figure 3 | Cell Death & Differentiation

Figure 3

From: The apoptotic members CD95, BclxL, and Bcl-2 cooperate to promote cell migration by inducing Ca2+ flux from the endoplasmic reticulum to mitochondria

Figure 3

BclxL-MT orchestrates CD95-mediated calcium influx in the mitochondrial matrix. Bclx-deficient MEFs (a) reconstituted with BclxL-WT (b), BclxL-ER (c), or BclxL-MT (d) were loaded with the mitochondrial Ca2+ probe (Rhod2, red) and cell-permeable Mitotracker (green) to label mitochondria. Left panels: confocal images of cells stimulated with cl-CD95L (100 ng/ml) for the indicated amount of time. Right panels: a region of interest was drawn in the Mitotracker-positive region (mitochondria) and fluorescence of the Ca2+ probe Rhod2 was monitored in this region. Graphs depict the overlay of the mitochondrial calcium response of 10 representative cells exposed to cl-CD95L. (e) The same experimental protocol was used as in (d), except that cells were pre-incubated for 1 h with a non-toxic dose of ABT-199 (1 μM). Images show t0, t0+35 s, t0+85 s, and t0+290 s of cells stimulated with cl-CD95L (100 ng/ml)

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