Figure 1
From: LH prevents cisplatin-induced apoptosis in oocytes and preserves female fertility in mouse
LH protects POs from degeneration induced by Cs. (a) Morphology of ovarian fragments from P4 ovaries of p-18 c-Kit/GFP mice cultured for 4 days (T0) and further 24 h of culture in the presence of the indicated compounds. GFP-positive POs (diameter <20 μm) constituted the large majority of the oocyte population in the cultured tissues and, unless otherwise indicated, the only type of oocytes scored in our assay; arrows indicate the few GFP-positive GOs (>20 μm). Scale bar 75 μm. (b) Percentage of healthy POs scored after 24 h culture with/out Cs and LH, mean±S.E.: Ctrl = 96 ± 1.2; 7.5 μM Cs=54±3.8; 10 μM Cs=33±1.1; 7.5 μM Cs±200 mIU LH=80±3.6; 10 μM Cs+200 mIU LH=68±3.0. The values represent the mean±S.E. of at least three independent experiments in which at least three fragments were scored for each experimental condition at T0 and after 24 h of culture. The same procedure and evaluation was used for the compounds and treatments described in all other experiments. (c) Percentage of healthy POs scored after 24 h of culture with/out Cs and FSH: Ctrl = 95 ± 1.1; 10 μM Cs=29±1.4; 10 μM Cs+400 mIU FSH=42±2.8. Statistical differences are versus Cs groups. *P<0.05, ***P<0.001
