Figure 2
From: LH prevents cisplatin-induced apoptosis in oocytes and preserves female fertility in mouse
Expression of LHR in ovaries and cultured ovarian tissue fragments. (a) Representative western blotting for LHR performed on samples obtained from ovaries at different developmental stages (NB=new born; P=days after birth) and ovarian fragments (Fr) from P4 ovaries cultured for 4 days in at least two different experiments; samples from adult ovaries (AO), adult testis (T) and MCF-7 cells, are also shown as positive control. (b) Representative RT-PCR for LHR transcripts performed on samples of denuded oocytes (DO) and ovarian somatic cells (SC) obtained from P4 ovaries. The analysis was repeated at least two times using different samples. (c and d) Immunolocalization of LHR in adult, P8 ovaries and P4 ovarian fragments cultured for 4 days, respectively; (d) right panel, a magnified image of the square region is shown. Scale bar 50 μm
