Table 1 Culture systems devised in the attempt to reconstitute various stages of mouse oogenesis in vitro

From: Complete in vitro oogenesis: retrospects and prospects

Germ cell sources

Culture method

Results

Year

References

12.5 dpc ovary

OC attached on plates

MII

2009

29

12.5 dpc ovary

OC Transwell membrane on+FC on Transwell-COL membrane+NT

Pups

2002

12

12.5 dpc ovary

OC on Transwell-COL membranes+FC on Millicell membrane+FC on Transwell-COL or Millicell membranes

Pups

2016

2

16.5 dpc ovary

OC on Millicell-PC membrane+ FC culture in droplets

Morulae-blastocysts

2007

45

Primordial follicles

OC on Millicell-PC membrane+FC Transwell-COL membrane

Pups

1996, 2003

11, 39

Primary/early secondary follicles

FC in Collagen gels+ on Transwell-COL membrane

Pups

2013

42

Oocytes from 12–14 dpp ovary

Oocytes co-cultured with PAGCs

Morula -blastocysts

2011

46

ES cells

Reconstituted ovaries+transplantation

Offspring

2012

17

ES cells

Reconstituted ovaries+IVDi +IVG

Offspring

2016

3

  1. Abbreviations: COL, collagen; FC, follicles culture; IVDi, in vitro differentiation; IVG, in vitro growth; MII, meiosis II; NT, nuclear transfer; OC, organ culture; PAGCs, preantral granulosa cell