Figure 2
Inflammatory phenotype of microglia induced by LPS or oxidative stress in vitro. Cultured BV2 cells were stimulated with either LPS (100 ng/ml), H2O2 (300 μM) or vehicle (PBS) for 24 h, respectively. (a and b) Cell lysates were prepared for western blotting. Increased expression levels of TNF-α, CCL2 and IL-17 were observed in LPS- and H2O2-stimulated cells. n=3 independent cultures. (c and d) Immunostaining results showed increased expression of pro-inflammatory cytokines (TNF-α and IL-17) in H2O2-treated cells than PBS-treated ones. CD11b and Iba-1 were used as microglia markers. Eight images were collected randomly in each culture and the experiments were repeated at least three times. Scale bar: 50 μm. **P<0.01; ***P<0.001
